Easy and cost-effective stable positioning of suspension cells during live-cell imaging
Main Article Content
Keywords
live-cell imaging, suspension culture, confocal laser scanning microscope
Abstract
Dynamic processes of cells can be best monitored when living cells are analyzed by imaging. While it is easy to observe adherent living cells it has been extremely challenging to analyze suspension cells. This cell type floats freely in the culture dish, and it is only a question of time when the focus or the observation field is lost. In order to keep the cells in focus, an easy and inexpensive method allowing the observation of living suspension cells during confocal laser scanning microscope imaging was developed.
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References
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3. Walston T, Hardin J (2010) An agar mount for observation of Caenorhabditis elegans embryos. Cold Spring Harb Protoc.2010(12):pdb prot5540. doi: 10.1101/pdb.prot5540. PubMed PMID: 21123427
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5. Ott C, Lippincott-Schwartz J (2012) Visualization of live primary cilia dynamics using fluorescence microscopy. Curr Protoc Cell Biol.Chapter 4:Unit 4 26. doi: 10.1002/0471143030.cb0426s57. PubMed PMID: 23208547
6. Arkwright PD, Luchetti F, Tour J, Roberts C, Ayub R, Morales AP, et al. (2010) Fas stimulation of T lymphocytes promotes rapid intercellular exchange of death signals via membrane nanotubes. Cell Res.20(1):72-88. doi: 10.1038/cr.2009.112. PubMed PMID: 19770844
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8. Zanetti-Domingues LC, Martin-Fernandez ML, Needham SR, Rolfe DJ, Clarke DT (2012) A systematic investigation of differential effects of cell culture substrates on the extent of artifacts in single-molecule tracking. PLoS One.7(9):e45655. doi: 10.1371/journal.pone.0045655. PubMed PMID: 23049831
9. Mattila PK, Lappalainen P (2008) Filopodia: molecular architecture and cellular functions. Nat Rev Mol Cell Biol.9(6):446-454. doi: 10.1038/nrm2406. PubMed PMID: 18464790
10. Jiang X, Zhang J, Huang Y (2015) Tetraspanins in cell migration. Cell Adh Migr.9(5):406-415. doi: 10.1080/19336918.2015.1005465. PubMed PMID: 26091149
11. Kofanova OA, Davis K, Glazer B, De Souza Y, Kessler J, Betsou F, et al. (2014) Viable mononuclear cell stability study for implementation in a proficiency testing program: impact of shipment conditions. Biopreserv Biobank.12(3):206-216. doi: 10.1089/bio.2013.0090. PubMed PMID: 24955735
12. Burd JF, Usategui-Gomez M (1973) A colorimetric assay for serum lactate dehydrogenase. Clin Chim Acta.46(3):223-227. PubMed PMID: 4725386
13. Korzeniewski C, Callewaert DM (1983) An enzyme-release assay for natural cytotoxicity. J Immunol Methods.64(3):313-320. PubMed PMID: 6199426
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Published
Oct 17, 2017
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Ivanusic D, Madela K, Denner J. Easy and cost-effective stable positioning of suspension cells during live-cell imaging. J Biol Methods [Internet]. 2017 Oct. 17 [cited 2024 Apr. 25];4(4):e80. Available from: https://jbmethods.org/index.php/jbm/article/view/203
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