Visualization of subdiffusive sites in a live single cell

Authors

  • Zeno Földes-Papp Head of the Department of Geriatrics, Asklepios Clinic Lindau, 88131 Lindau (at Lake Constance), Bavaria, Germany
  • Gerd Baumann Head of the Mathematics Department, Faculty of Basic Sciences, German University in Cairo (GUC), 11835 New Cairo City, Egypt
  • Long-Cheng Li Ractigen Therapeutics, Jiangsu, Nantong, 226400, China and Institute of Reproductive Medicine, School of Medicine, Nantong University, Nantong, 226001, China

DOI:

https://doi.org/10.14440/jbm.2021.348

Keywords:

anomalous diffusion, complex diffusion mechanism, imaging, live cell, number of traps

Abstract

We measured anomalous diffusion in human prostate cancer cells which were transfected with the Alexa633 fluorescent RNA probe and co-transfected with enhanced green fluorescent protein-labeled argonaute2 protein by laser scanning microscopy. The image analysis arose from diffusion based on a

Author Biography

Zeno Földes-Papp, Head of the Department of Geriatrics, Asklepios Clinic Lindau, 88131 Lindau (at Lake Constance), Bavaria, Germany

Visiting Professor of Medical Biochemistry, German University Cairo (GUC)

References

Jonkman J, Brown CM, Wright GD, Anderson KI, North AJ (2020) Tutorial: guidance for quantitative confocal microscopy. Nat Protoc 15 (5): 1585-1611.

Ward EN, Pal R (2017) Image scanning microscopy: an overview. J Microsc 266 (2): 221-228.

Weisshart K, J

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Published

2021-01-30

How to Cite

1.
Földes-Papp Z, Baumann G, Li L-C. Visualization of subdiffusive sites in a live single cell. J Biol Methods [Internet]. 2021Jan.30 [cited 2021Apr.12];8(1):e142. Available from: https://jbmethods.org/index.php/jbm/article/view/348

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Articles