Concentrating white spot syndrome virus by alum for field detection using a monoclonal antibody based flow-through assay

Authors

  • Amrita Rani Aquatic Animal Health Laboratory, College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University
  • Satish R. Poojary Aquatic Animal Health Laboratory, College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University
  • Naveenkumar B. Tammegouda Aquatic Animal Health Laboratory, College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University
  • Abhiman P. Ballaya Aquatic Animal Health Laboratory, College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University
  • Prakash Patil Aquatic Animal Health Laboratory, College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University
  • Ramesh K. Srinivasayya Aquatic Animal Health Laboratory, College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University
  • Shankar M. Kalkuli Aquatic Animal Health Laboratory, College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University
  • Santhosh K. Shivakumaraswamy Department of Fishery Microbiology, College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University

DOI:

https://doi.org/10.14440/jbm.2016.105

Keywords:

alum, field detection, flocculation, flow-through assay, WSSV

Abstract

A simple and easy method of concentrating white spot syndrome virus by employing aluminium sulphate, alum as a flocculant was developed and evaluated for field detection. The concentrated virus was detected by a monoclonal antibody based flow-through assay, RapiDot and compared its performance with polymerase chain reaction. The semi-purified virus that was flocculated by 15 and 30 ppm alum in a 50 ml cylinder can be detected successfully by both RapiDot and I step PCR. In addition, alum could also flocculate the virus that is detectable by II step PCR and the concentration of virus was similar to the one observed in water from an infected pond. Furthermore, experimental infection studies validated the successful concentration of virus by alum flocculation followed by rapid detection of virus using monoclonal antibody based RapiDot. Overall, the results obtained in this study indicate that the white spot syndrome virus in water could be easily flocculated by alum for sensitive field detection by monoclonal antibody based RapiDot.

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Published

2016-05-25

How to Cite

1.
Rani A, Poojary SR, Tammegouda NB, Ballaya AP, Patil P, Srinivasayya RK, Kalkuli SM, Shivakumaraswamy SK. Concentrating white spot syndrome virus by alum for field detection using a monoclonal antibody based flow-through assay. J Biol Methods [Internet]. 2016May25 [cited 2021Jun.16];3(2):e39. Available from: https://jbmethods.org/jbm/article/view/105

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