A stepwise procedure for isolation of murine bone marrow and generation of dendritic cells

Authors

  • Alka Madaan Dabur Research Foundation 22, Site-IV, Sahibabad, Ghaziabad, Uttar Pradesh 201010 INDIA
  • Ritu Verma Cell Biology Lab, Dabur Research Foundation, 22, Site IV, Sahibabad, Ghaziabad, Uttar Pradesh-201010, India
  • Anu T Singh Cell Biology Lab, Dabur Research Foundation, 22, Site IV, Sahibabad, Ghaziabad, Uttar Pradesh-201010, India
  • Swatantra Kumar Jain Department of Biotechnology, Jamia Hamdard, New Delhi-110062, India
  • Manu Jaggi Cell Biology Lab, Dabur Research Foundation, 22, Site IV, Sahibabad, Ghaziabad, Uttar Pradesh-201010, India

DOI:

https://doi.org/10.14440/jbm.2014.12

Keywords:

Bone marrow, dendritic cells, primary culture

Abstract

Bone marrow derived Dendritic cells (BMDCs) are routinely employed in cell based assays to evaluate immunomodulatory and anti-inflammatory activities. Hence, simplified, stepwise, defined and standardized methods are required for isolation of bone marrow cells from mice, propagating them in presence of growth factors and obtaining high and reproducible yields of BMDCs. Here, we describe a detailed, stepwise protocol with pictorial representation to isolate bone marrow from mouse femur and development of dendritic cells. Mouse bone marrow cells are cultured in presence of granulocyte-macrophage colony stimulating factor (GM-CSF) for 6 days to generate BMDCs.

Author Biography

Alka Madaan, Dabur Research Foundation 22, Site-IV, Sahibabad, Ghaziabad, Uttar Pradesh 201010 INDIA

Senior Research Scientist 

Cell Biology Lab

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Published

2014-02-07

How to Cite

1.
Madaan A, Verma R, Singh AT, Jain SK, Jaggi M. A stepwise procedure for isolation of murine bone marrow and generation of dendritic cells. J Biol Methods [Internet]. 2014Feb.7 [cited 2022Jan.22];1(1):e1. Available from: https://jbmethods.org/jbm/article/view/12

Issue

Section

Protocols