Patient-derived xenograft model for uterine leiomyoma by sub-renal capsule grafting

Authors

  • Takeshi Kurita Ohio State University
  • Vanida Ann Serna Ohio State University

DOI:

https://doi.org/10.14440/jbm.2018.243

Keywords:

estradiol, fibroids, myometrium, NSG mouse, patient-derived xenografts, preclinical model, progesterone

Abstract

Uterine leiomyoma (UL) or fibroid is a benign smooth muscle tumor of the myometrium with a lifetime incidence of approximately 70%. ULs often require medical intervention due to severe symptoms such as heavy menstrual bleeding and abdominal pain. Although the most common and effective management of ULs is surgical removal, the invasive surgical procedure imposes physical and psychological burdens on the patients. Moreover, the economic burden of UL on health care system is enormous due to the high cost of surgeries. Thus, therapeutic options with long-term efficacy to replace surgical management are urgently needed. For the development of such medical options, reliable preclinical research models are imperative. Ex vivo culture of UL cells has been the primary research model for decades. However, recent studies demonstrated that primary cell culture is not a suitable model for UL research, as primary cultures of ULs mostly consist of non-tumor fibroblasts. Here we describe the protocol for patient-derived xenograft of UL, which faithfully replicates the phenotypes of human UL in situ.

Author Biography

Takeshi Kurita, Ohio State University

Associate professor, Department of Cancer Biology and Genetics, The Comprehensive Cancer Center

References

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Published

2018-04-16

How to Cite

1.
Kurita T, Serna VA. Patient-derived xenograft model for uterine leiomyoma by sub-renal capsule grafting. J Biol Methods [Internet]. 2018Apr.16 [cited 2022Aug.11];5(2):e91. Available from: https://jbmethods.org/jbm/article/view/243

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Section

Protocols