A simple method for long-term vital-staining of ciliated epidermal cells in aquatic larvae

Authors

  • Jörn von Döhren Institute of Evolutionary Biology and Ecology, University of Bonn
  • Sabrina Kuhl Institute of Evolutionary Biology and Ecology, University of Bonn

DOI:

https://doi.org/10.14440/jbm.2020.320

Keywords:

cLSM, DiI, fluorescent staining, prototroch, Spiralia

Abstract

Observing the process of growth and differentiation of tissues and organs is of crucial importance for the understanding of the evolution of organs in animals. Unfortunately, it is notoriously difficult to continuously monitor developmental processes due to the extended time they take. Long-term labeling of the tissues of interest represents a promising alternative to raise these pivotal data. In the case of the prototroch, a band of ciliated cells typical of marine, planktotrophic trochophora larvae, we were able to apply a long-term fluorescent vital-staining to the prototroch cells that remains detectable throughout further larval life. We were able to stain ciliated cells of planktonic larvae from different spiralian clades by using long-chain dialkylcarbocyanine dyes that are detectable in different fluorescent emission spectra in combination with a non-ionic surfactant. The larvae survived and developed normally, their ciliated cells retaining the originally applied fluorescent labels. Combined with additional fluorescent staining of the larvae after fixation, we provide an easy, versatile, and broadly applicable method to investigate the processes of the differentiation of epidermal organs in various aquatic larvae.

Author Biography

Jörn von Döhren, Institute of Evolutionary Biology and Ecology, University of Bonn

Dr. at the Institute of Evolutionary Biology and Ecology of the University of Bonn

References

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Published

2020-04-29

How to Cite

1.
von Döhren J, Kuhl S. A simple method for long-term vital-staining of ciliated epidermal cells in aquatic larvae. J Biol Methods [Internet]. 2020Apr.29 [cited 2022Aug.11];7(2):e132. Available from: https://jbmethods.org/jbm/article/view/320

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