Expansion and cellular characterization of primary human adherent cells in the Quantum® Cell Expansion System, a hollow-fiber bioreactor system


  • Boah Vang Terumo BCT Inc
  • Nathan Frank Terumo BCT Inc
  • Mark Jones Terumo BCT Inc
  • Brian Nankervis Terumo BCT Inc
  • Claire Coeshott Terumo BCT Inc




adherent cells, fibroblasts, mesenchymal stem/stromal cells, myoblasts, Quantum cell expansion system


Primary adherent cell types can be expanded in the Quantum® Cell Expansion System (Quantum system), an automated platform that utilizes a hollow-fiber bioreactor. This system can replace manual cell culture and produce cells that retain their phenotypes and functionality. Bone- marrow-derived and adipose-derived mesenchymal stem/stromal cells have previously been successfully expanded on the Quantum system. We have now successfully used the Quantum system to expand fibroblasts and myoblasts. Hollow-fiber bioreactors were coated with adherence-supporting proteins, and then cells were loaded and expanded in the appropriate growth medium for 7 to 15 d. Cells were harvested from the bioreactors using enzymatic reagents. Harvested cell yields ranged from 100 × 106 to 1 × 109 cells, with viability typically above 90%. The number of doublings obtained from Quantum system harvests ranged from 4 to 9. The Quantum system is a functionally closed expansion system that can reduce contamination due to minimal interventions and can automate the culture process to reduce labor and reagent costs.



Mao AS, Mooney DJ (2015) Regenerative medicine: current therapies and future directions. Proc Natl Acad Sci USA 112(47): 14452-14459.

Ballas CB, Zielske SP, Gerson SL (2002) Adult bone marrow stem cells for cell and gene therapies: implications for greater use. J Cell Biochem Suppl 38: 20-28.

Alberts B, Johnson A, Lewis J, et al. Molecular Biology of the Cell. 4th edition. New York: Garland Science; 2002. Fibroblasts and Their Transformations: The Connective-Tissue Cell Family. Available from: https://www.ncbi.nlm.nih.gov/books/NBK26889.

Durrani S, Konoplyannikov M, Ashraf M, Haider KH (2010) Skeletal myoblasts for cardiac repair. Regen Med 5(6): 919-932.

Choi W, Kwon SJ, Jin HJ, et al. (2017) Optimization of culture conditions for rapid clinical-scale expansion of human umbilical cord blood-derived mesenchymal stem cells. Clin Transl Med 6(1): 38.

Hanley PJ, Mei Z, Durett AG, et al. (2014) Efficient manufacturing of therapeutic mesenchymal stromal cells with the use of the Quantum Cell Expansion System. Cytotherapy 16(8): 1048-1058.

Barckhausen C, Rice B, Baila S, et al. (2016) GMP-Compliant Expansion of Clinical-Grade Human Mesenchymal Stromal/Stem Cells Using a Closed Hollow Fiber Bioreactor. Methods Mol Biol 1416: 389-412.

Jones M, Varella-Garcia M, Skokan M, et al. (2013) Genetic stability of bone marrow-derived human mesenchymal stromal cells in the Quantum System. Cytotherapy 15(11): 1323-1339.

Haack-Sorensen M, Follin B, Juhl M, et al. (2016) Culture expansion of adipose derived stromal cells. A closed automated Quantum Cell Expansion System compared with manual flask-based culture. J Transl Med 14(1): 319.

Jarocha D, Stangel-Wojcikiewicz K, Basta A, Majka M (2014) Efficient myoblast expansion for regenerative medicine use. Int J Mol Med 34(1): 83-91.

Del Pino A, Ligero G, Lopez MB, et al. (2015) Morphology, cell viability, karyotype, expression of surface markers and plasticity of three primary cell line cultures before and after the cryostorage in LN2 and GN2. Cryobiology 70(1): 1-8.

Berendse M, Grounds MD, Lloyd CM (2003) Myoblast structure affects subsequent skeletal myotube morphology and sarcomere assembly. Exp Cell Res 291(2): 435-450.

Nehlin JO, Just M, Rustan AC (2011) Human myotubes from myoblast cultures undergoing senescence exhibit defects in glucose and lipid metabolism. Biogerontology 12: 349-365.

Coeshott C, Vang B, Jones M, Nankervis B (2019) Large-scale expansion and characterization of CD3+ T-cells in the Quantum® Cell Expansion System. J Transl Med 17(1): 258.

Russell AL, Lefavor RC, Zubair AC (2018) Characterization and cost-benefit analysis of automated bioreactor-expanded mesenchymal stem cells for clinical applications. Transfusion 58(10): 2374-2382.




How to Cite

Vang B, Frank N, Jones M, Nankervis B, Coeshott C. Expansion and cellular characterization of primary human adherent cells in the Quantum® Cell Expansion System, a hollow-fiber bioreactor system. J Biol Methods [Internet]. 2020Apr.8 [cited 2022Aug.11];7(2):e130. Available from: https://jbmethods.org/jbm/article/view/329



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