Expansion and cellular characterization of primary human adherent cells in the Quantum® Cell Expansion System, a hollow-fiber bioreactor system

Authors

  • Boah Vang Terumo BCT Inc
  • Nathan Frank Terumo BCT Inc
  • Mark Jones Terumo BCT Inc
  • Brian Nankervis Terumo BCT Inc
  • Claire Coeshott Terumo BCT Inc

DOI:

https://doi.org/10.14440/jbm.2020.329

Keywords:

adherent cells, fibroblasts, mesenchymal stem/stromal cells, myoblasts, Quantum cell expansion system

Abstract

Primary adherent cell types can be expanded in the Quantum® Cell Expansion System (Quantum system), an automated platform that utilizes a hollow-fiber bioreactor. This system can replace manual cell culture and produce cells that retain their phenotypes and functionality. Bone- marrow-derived and adipose-derived mesenchymal stem/stromal cells have previously been successfully expanded on the Quantum system. We have now successfully used the Quantum system to expand fibroblasts and myoblasts. Hollow-fiber bioreactors were coated with adherence-supporting proteins, and then cells were loaded and expanded in the appropriate growth medium for 7 to 15 d. Cells were harvested from the bioreactors using enzymatic reagents. Harvested cell yields ranged from 100 × 106 to 1 × 109 cells, with viability typically above 90%. The number of doublings obtained from Quantum system harvests ranged from 4 to 9. The Quantum system is a functionally closed expansion system that can reduce contamination due to minimal interventions and can automate the culture process to reduce labor and reagent costs.

References

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Published

2020-04-08

How to Cite

1.
Vang B, Frank N, Jones M, Nankervis B, Coeshott C. Expansion and cellular characterization of primary human adherent cells in the Quantum® Cell Expansion System, a hollow-fiber bioreactor system. J Biol Methods [Internet]. 2020Apr.8 [cited 2022Aug.11];7(2):e130. Available from: https://jbmethods.org/jbm/article/view/329

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Section

Application Notes