Advanced methods of microscope control using μManager software
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Keywords
high-speed imaging, high-content screening, localization microscopy, Micro-Manager, open-source microscopy
Abstract
µManager is an open-source, cross-platform desktop application, to control a wide variety of motorized microscopes, scientific cameras, stages, illuminators, and other microscope accessories. Since its inception in 2005, µManager has grown to support a wide range of microscopy hardware and is now used by thousands of researchers around the world. The application provides a mature graphical user interface and offers open programming interfaces to facilitate plugins and scripts. Here, we present a guide to using some of the recently added advanced µManager features, including hardware synchronization, simultaneous use of multiple cameras, projection of patterned light onto a specimen, live slide mapping, imaging with multi-well plates, particle localization and tracking, and high-speed imaging.
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References
Micro-Manager website. Micro-Manager team in Ron Vale lab, Dept. of Cellular and Mol. Pharm., University of California San Francisco. Cited on August 18, 2014. http://micro-manager.org.
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Devices supported in µManager. Micro-Manager team in Ron Vale lab, Dept. of Cellular and Mol. Pharm., University of California San Francisco. Cited on August 18, 2014. http://micro-manager.org/wiki/Device Support
ImageJ plugin GridAligner. Nico Stuurman in Ron Vale Lab, Dept. of Cellular and Mol. Pharm., University of California San Francisco. Cited on August 18, 2014. http://valelab.ucsf.edu/~nstuurman/IJplugins/GridAligner.html
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