Triatomines intrahaemocoelic inoculation protocol: a useful tool to check infectivity in insects
Main Article Content
Keywords
Chagas disease, inoculation protocol, intrahaemocoelic inoculation, Triatoma virus, Triatomines
Abstract
Vectors of Chagas disease are currently controlled by employing several chemical insecticides though there is a continuing search for alternative ecological methods against disease causing vectors. An effective method includes the use of specific pathogens as biological control agents. The aim of this work was to describe a complete experimental inoculation protocol in triatomines. The intrahaemocoelic inoculation technique can be applied to inoculate different kinds of microorganisms such as viruses, fungi, bacteria and protozoa; so it could be considered a useful tool in infective bioassays. This article includes results from evaluations of Triatoma virus (TrV, Dicistroviridae: Cripavirus) infectivity in several triatomine species. The protocol, also suitable for any other kind of insects, describes the materials and steps required to safely inoculate the insects, preventing any damage and/or contamination.
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References
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7. Galvao, C, Angulo, VM (2006) Belminus corredori, a new species of Bolboderinig (Hemiptera: Reduviidae: Triatominae) from Santander, Colombia. Zootaxa. 1241:61–68.
8. Guhl F, Aguilera G, Pinto N, Vergara D (2007) Actualización de la distribución geográfica y ecoepidemiología de la fauna de triatominos (Reduviidae: Triatominae) en Colombia. Biomédica 27 supl. 1: 143-62.
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10. Muscio OA, La Torre JL, Scodeller EA (1987) Small Nonoccluded Viruses from Triatomine Bug Triatoma infestans (Hemiptera: Reduviidae). J Invertebr Pathol 49: 218-220,
11. Lecuona RE, Edelstein JD, Berretta MF, La Rossa FR, Arcas JA (2001) Evaluation of Bauveria bassiana (Hyphomicetes) strains as potential agents for control of Triatoma infestans (Hemiptera: Reduvidae). J Med Entomol 38: 172-179.
12. Luz C, Rocha LFN, Nery GV (2004) Detection of Entomopathogenic Fungi in Peridomestic Triatomine-Infested Areas in Central Brazil and Fungal Activity Against Triatoma infestans (Klug) (Hemiptera: Reduvidae) Neotrop Entomol 33 (6) 783-791.
13. Muscio OA, La Torre JL, Scodeller EA (1988) Characterization of Triatoma virus, a Picorna-like Virus isolated from the Triatominae bug Triatoma infestans. J Gen Virol 69: 2929-2934.
14. Muscio OA, La Torre JL, Bonder MA, Scodeller EA (1997) Triatoma virus pathogenicity in laboratory colonies of Triatoma infestans (Hemiptera: Rduviidae). J Med Entomol 34: 253-256.
15. Rozas Dennis GS, Cazzaniga NJ (2000) Effects of Triatoma virus (TrV) on the fecundity and moulting of Triatoma infestans (Hemiptera:Reduviidae). Ann of Trop Med & Parasitology 94: 633-641.
16. Rozas Dennis GS, Cazzaniga NJ, Guérin DMA (2002) Triatoma patagonica (Hemiptera, Reduviidae), a new host for Triatoma virus (TrV)”. Mem Inst Oswaldo Cruz 97: 427- 429.
17. Marti GA, Echeverria MG, Susevich ML, Becnel JJ, Pelizza SA, et al. (2009) Prevalence and distribution of parasites and pathogens of Triatominae from Argentina, with emphasis on Triatoma infestans and Triatoma virus TrV. J Invertebr Pathol 102: 233-237.
18. Susevich ML, Marti G, Serena MS, Echeverria MG (2012) New Triatoma virus hosts in wild habitats of Argentina. J Invertebr Pathol 110: 405–407.
19. Marti GA, Echeverría MG, Susevich ML, Ceccarelli S, Balsalobre A, Rabinovich JE, Diotaiuti L and Guérin DMA (2013) Exploration for Triatoma virus (TrV) infection in laboratory-reared triatomines of Latin America: a collaborative study International. J Trop Insect Science 33: 294-304.
20. González NL, (2008) “Determinación del efecto de la infección con Triatoma virus en diferentes especies de triatominos. Tesis de Maestría, Universidad de los Andes, Bogotá Colombia.
21. Ceccarelli S, Balsalobre A, Susevich ML, Gorla DE, Marti GA. 2015. Modelling the potential geographic distribution of triatomines infected by Triatoma virus in the southern cone of South America. Parasites & Vectors 8:153
22. Rozas Dennis GS, La Torre JL, Muscio OA, Guérin DMA (2000) “Direct Methods for detecting picorna-like virus from dead and alive triatomine insects”. Mem Inst Oswaldo Cruz 95: 323-327.
23. Higgs S, Beaty BJ (1996) Rearing and Containment of mosquito vectors. In: Beaty BJ, Marquardt WC, editors. The Biology of Disease Vectors: Chapter 35, University Press of Colorado. pp. 595-605.
2. WHO/2013. Department of control of neglected tropical diseases. Sustaining the drive to overcome the global impact of neglected tropical diseases, Second WHO report on neglected tropical diseases. Ed. Prof. David WT Crompton. 140p.WHO/HTM/NTD/2013.1.
3. Schofield CJ (1994) Triatominae. Biología y Control. United Kingdom ZENECA Salud Pública Eurocommunica Publications 80 p.
4. Nunes MC, Dones W, Morillo CA, Encina JJ, Ribeiro, AL (2013) Council on Chagas Disease of the Interamenrican Society of Cardiology. J Am Coll Cardiol 62: 767-76.
5. Zeledón R, Rabinovich JE (1981) Chagas disease: an ecological appraisal with special emphasis on its insect vectors. Ann Rev Entomol 26: 101-133.
6. Wisnivesky-Colli C, Schweigmann NJ, Pietrokovosky S, Bottazi V, Rabinovich JE (1997). Spatial distribution of Triatoma guasayana (Hemiptera: Reduviidae) in hardwood forest biotopes in Santiago del Estero, Argentina. J Med Entomol 34: 102-109.
7. Galvao, C, Angulo, VM (2006) Belminus corredori, a new species of Bolboderinig (Hemiptera: Reduviidae: Triatominae) from Santander, Colombia. Zootaxa. 1241:61–68.
8. Guhl F, Aguilera G, Pinto N, Vergara D (2007) Actualización de la distribución geográfica y ecoepidemiología de la fauna de triatominos (Reduviidae: Triatominae) en Colombia. Biomédica 27 supl. 1: 143-62.
9. Sandoval, CM, Pabon, E, Jurberg, J, Galvao, C (2007). Belminus ferroae n. sp. from the Colombian north-east, with a key to the species of the genus (Hemiptera Reduviidae: Triatominae). Zootaxa 1443:55-64
10. Muscio OA, La Torre JL, Scodeller EA (1987) Small Nonoccluded Viruses from Triatomine Bug Triatoma infestans (Hemiptera: Reduviidae). J Invertebr Pathol 49: 218-220,
11. Lecuona RE, Edelstein JD, Berretta MF, La Rossa FR, Arcas JA (2001) Evaluation of Bauveria bassiana (Hyphomicetes) strains as potential agents for control of Triatoma infestans (Hemiptera: Reduvidae). J Med Entomol 38: 172-179.
12. Luz C, Rocha LFN, Nery GV (2004) Detection of Entomopathogenic Fungi in Peridomestic Triatomine-Infested Areas in Central Brazil and Fungal Activity Against Triatoma infestans (Klug) (Hemiptera: Reduvidae) Neotrop Entomol 33 (6) 783-791.
13. Muscio OA, La Torre JL, Scodeller EA (1988) Characterization of Triatoma virus, a Picorna-like Virus isolated from the Triatominae bug Triatoma infestans. J Gen Virol 69: 2929-2934.
14. Muscio OA, La Torre JL, Bonder MA, Scodeller EA (1997) Triatoma virus pathogenicity in laboratory colonies of Triatoma infestans (Hemiptera: Rduviidae). J Med Entomol 34: 253-256.
15. Rozas Dennis GS, Cazzaniga NJ (2000) Effects of Triatoma virus (TrV) on the fecundity and moulting of Triatoma infestans (Hemiptera:Reduviidae). Ann of Trop Med & Parasitology 94: 633-641.
16. Rozas Dennis GS, Cazzaniga NJ, Guérin DMA (2002) Triatoma patagonica (Hemiptera, Reduviidae), a new host for Triatoma virus (TrV)”. Mem Inst Oswaldo Cruz 97: 427- 429.
17. Marti GA, Echeverria MG, Susevich ML, Becnel JJ, Pelizza SA, et al. (2009) Prevalence and distribution of parasites and pathogens of Triatominae from Argentina, with emphasis on Triatoma infestans and Triatoma virus TrV. J Invertebr Pathol 102: 233-237.
18. Susevich ML, Marti G, Serena MS, Echeverria MG (2012) New Triatoma virus hosts in wild habitats of Argentina. J Invertebr Pathol 110: 405–407.
19. Marti GA, Echeverría MG, Susevich ML, Ceccarelli S, Balsalobre A, Rabinovich JE, Diotaiuti L and Guérin DMA (2013) Exploration for Triatoma virus (TrV) infection in laboratory-reared triatomines of Latin America: a collaborative study International. J Trop Insect Science 33: 294-304.
20. González NL, (2008) “Determinación del efecto de la infección con Triatoma virus en diferentes especies de triatominos. Tesis de Maestría, Universidad de los Andes, Bogotá Colombia.
21. Ceccarelli S, Balsalobre A, Susevich ML, Gorla DE, Marti GA. 2015. Modelling the potential geographic distribution of triatomines infected by Triatoma virus in the southern cone of South America. Parasites & Vectors 8:153
22. Rozas Dennis GS, La Torre JL, Muscio OA, Guérin DMA (2000) “Direct Methods for detecting picorna-like virus from dead and alive triatomine insects”. Mem Inst Oswaldo Cruz 95: 323-327.
23. Higgs S, Beaty BJ (1996) Rearing and Containment of mosquito vectors. In: Beaty BJ, Marquardt WC, editors. The Biology of Disease Vectors: Chapter 35, University Press of Colorado. pp. 595-605.
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Published
May 23, 2016
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How to Cite
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Rozas-Dennis GS, Marti GA, González NL, Guhl F, Guérin DM. Triatomines intrahaemocoelic inoculation protocol: a useful tool to check infectivity in insects. J Biol Methods [Internet]. 2016 May 23 [cited 2024 Apr. 26];3(2):e40. Available from: https://jbmethods.org/index.php/jbm/article/view/46
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