Development of an In Situ Hybridization Method for Detection of Akabane Virus

Main Article Content

Nihat Toplu
Tuba Çiğdem Oğuzoğlu
Ayşe Nur Akkoç

Keywords

Akabane virus, in situ hybridization, method, pathology

Abstract

Akabane virus (AKAV) is an arbovirus belonging to the family Bunyaviridae, genus Orthobunyavirus. AKAV consists of three-segment (L, M, and S RNA segments), negative single-stranded RNA. The aim of this study was to investigate an in situ hybridization method (ISH) in a Vero E6 cell line infected with Akabane virus. The 320 base pair amplicon was obtained by RT-PCR with a primer pair and labeled with digoxigenin. Akabane virus RNAs were seen as a granular pattern in the cytoplasm of infected cells. As a result, the expression of the particular Akabane virus gene area was successfully disclosed in the current investigation using the ISH method with a digoxigenin-labeled probe.

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