Novel adaptation of a running suture technique in a mouse model of corneal transplantation


  • Hayate Nakagawa Schepens Eye Research Institute, Massachusetts Eye and Ear, Department of Ophthalmology, Harvard Medical School
  • Tomas Blanco
  • Francesca Kahale
  • Rohan Bir Singh
  • Thomas H. Dohlman
  • Reza Dana


animal model, corneal transplantation, running suture technique


Several murine models of corneal transplantation have been developed over the years to study the immunopathological processes that lead to the failure of grafted corneas. In all of them, the classic eight interrupted sutures technique is utilized for transplanting the donor cornea on the host bed. However, in clinical practice, a single continuous suture with a single knot is generally performed for corneal transplantation. Here, we describe the adaptation of the single continuous suture technique in a mouse model of corneal transplantation.


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How to Cite

Nakagawa H, Blanco T, Kahale F, Singh RB, Dohlman TH, Dana R. Novel adaptation of a running suture technique in a mouse model of corneal transplantation. J Biol Methods [Internet]. 2021Oct.22 [cited 2022Jan.27];8(4):e156. Available from:




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