Triatomines intrahaemocoelic inoculation protocol: a useful tool to check infectivity in insects


  • Gabriela S. Rozas-Dennis Departamento de Biología, Bioquímica y Farmacia, Universidad Nacional del Sur
  • Gerardo A. Marti Centro de Estudios Parasitológicos y de Vectores (CEPAVE CCT-La Plata-CONICET-UNLP)
  • Nadia Lorena González Departamento de Ciencias Biológicas, Centro de Investigaciones en Microbiología y Prasitología Tropical. Universidad de los Andes, Bogotá, Colombia
  • Felipe Guhl Departamento de Ciencias Biológicas. Centro de Investigaciones en Microbiología y Parasitología Tropical, Universidad de los Andes, Bogotá, Colombia
  • Diego M.A. Guérin Departamento de Bioquímica y Biología Molecular, Facultad de Ciencia y Tecnología. Universidad del Pais Vasco (EHU), Unidad de Biofísica (UBF,CSIC,UPV/EHU), Bizcaia, Spain



Chagas disease, inoculation protocol, intrahaemocoelic inoculation, Triatoma virus, Triatomines


Vectors of Chagas disease are currently controlled by employing several chemical insecticides though there is a continuing search for alternative ecological methods against disease causing vectors. An effective method includes the use of specific pathogens as biological control agents. The aim of this work was to describe a complete experimental inoculation protocol in triatomines. The intrahaemocoelic inoculation technique can be applied to inoculate different kinds of microorganisms such as viruses, fungi, bacteria and protozoa; so it could be considered a useful tool in infective bioassays. This article includes results from evaluations of Triatoma virus (TrV, Dicistroviridae: Cripavirus) infectivity in several triatomine species. The protocol, also suitable for any other kind of insects, describes the materials and steps required to safely inoculate the insects, preventing any damage and/or contamination.







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How to Cite

Rozas-Dennis GS, Marti GA, González NL, Guhl F, Guérin DM. Triatomines intrahaemocoelic inoculation protocol: a useful tool to check infectivity in insects. J Biol Methods [Internet]. 2016May23 [cited 2021Oct.15];3(2):e40. Available from: